5EEX
RADIATION DAMAGE TO THE TRAP-RNA COMPLEX: DOSE (DWD) 9.02 MGy
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-05-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.940 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 141.040, 111.050, 138.050 |
Unit cell angles | 90.00, 117.40, 90.00 |
Refinement procedure
Resolution | 58.601 - 1.980 |
R-factor | 0.214 |
Rwork | 0.212 |
R-free | 0.24480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5eeu |
RMSD bond length | 0.024 |
RMSD bond angle | 2.276 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.3.11) |
Phasing software | PHASES |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 62.610 | 62.610 | 2.010 |
High resolution limit [Å] | 1.980 | 10.840 | 1.980 |
Rmerge | 0.103 | 0.025 | 1.227 |
Rpim | 0.063 | 0.016 | 0.765 |
Total number of observations | 472756 | 2856 | 21594 |
Number of reflections | 130454 | ||
<I/σ(I)> | 8.8 | 26.5 | 1.1 |
Completeness [%] | 99.3 | 95.1 | 98.1 |
Redundancy | 3.6 | 3.5 | 3.4 |
CC(1/2) | 0.997 | 0.999 | 0.610 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293.15 | Potassium phosphate,L-tryptophan,potassium glutamate,triethanolamine,MgCl2,monomethyl ether PEG 2000 |