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5EEW

RADIATION DAMAGE TO THE TRAP-RNA COMPLEX: DOSE (DWD) 6.45 MGy

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE ID14-4
Synchrotron siteESRF
BeamlineID14-4
Temperature [K]100
Detector technologyCCD
Collection date2010-05-08
DetectorADSC QUANTUM 315r
Wavelength(s)0.940
Spacegroup nameC 1 2 1
Unit cell lengths141.040, 111.020, 137.990
Unit cell angles90.00, 117.41, 90.00
Refinement procedure
Resolution58.578 - 1.980
R-factor0.2106
Rwork0.209
R-free0.24320
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)5eeu
RMSD bond length0.024
RMSD bond angle2.276
Data reduction softwareMOSFLM
Data scaling softwareAimless (0.3.11)
Phasing softwarePHASER
Refinement softwarePHENIX
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]62.60062.6002.010
High resolution limit [Å]1.98010.8401.980
Rmerge0.0970.0231.053
Rpim0.0590.0150.657
Total number of observations472091283421737
Number of reflections130317
<I/σ(I)>9.327.11.2
Completeness [%]99.395.198.1
Redundancy3.63.53.4
CC(1/2)0.9970.9980.731
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.8293.15Potassium phosphate,L-tryptophan,potassium glutamate,triethanolamine,MgCl2,monomethyl ether PEG 2000

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