5EEO
soybean lipoxygenase(L1)-T756R
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-10 |
Detector | RIGAKU SATURN A200 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.708, 62.361, 92.351 |
Unit cell angles | 90.00, 103.25, 90.00 |
Refinement procedure
Resolution | 38.700 - 2.100 |
R-factor | 0.1892 |
Rwork | 0.186 |
R-free | 0.25440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1f8n |
RMSD bond length | 0.007 |
RMSD bond angle | 1.137 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.700 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.108 | 0.448 |
Number of reflections | 45398 | |
<I/σ(I)> | 28.4 | 7.2 |
Completeness [%] | 98.8 | 100 |
Redundancy | 5.8 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Lithium chloride, 20%(w/v) Polyethylene glycol 3,350 |