5EE1

Crystal structure of OsYchF1 at pH 7.85

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	160
Detector technology	CCD
Collection date	2015-05-12
Detector	ADSC QUANTUM 270
Wavelength(s)	1
Spacegroup name	P 2 21 21
Unit cell lengths	56.037, 70.394, 117.372
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.550
R-factor	0.23116
Rwork	0.229
R-free	0.26285
RMSD bond length	0.010
RMSD bond angle	1.345
Data reduction software	MAR345dtb
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.550
Number of reflections	15955
<I/σ(I)>	17.2
Completeness [%]	99.7
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.85	277	PEG