5EDX
Crystal structure of swine CD8aa homodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-08 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 80.965, 80.965, 95.194 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.909 - 1.801 |
| R-factor | 0.184 |
| Rwork | 0.183 |
| R-free | 0.20880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cd8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.997 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.107 | |
| Number of reflections | 33859 | |
| <I/σ(I)> | 30.235 | 5.476 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 15.2 | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 1.0 M ammonium sulfate, 0.1 M Bis-Tris pH 5.5, 1% (w/v) polyethylene glycol 3350 |
