5EDU
Crystal structure of human histone deacetylase 6 catalytic domain 2 in complex with trichostatin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.262, 149.032, 216.333 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 87.540 - 2.790 |
| R-factor | 0.2157 |
| Rwork | 0.213 |
| R-free | 0.27540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4cbt |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.738 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.3_1479) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 149.000 | |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.204 | 1.853 |
| Number of reflections | 40864 | |
| <I/σ(I)> | 12.9 | 1.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M potassium sodium tartrate, 20% PEG3350 |
