5ED8

Crystal structure of CC2-SUN of mouse SUN2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-11
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	63.374, 63.374, 192.786
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.940 - 2.500
R-factor	0.2157
Rwork	0.214
R-free	0.25450
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.729
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXCD
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	14373
<I/σ(I)>	21.6
Completeness [%]	99.5	100
Redundancy	4.3	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	289	1.4M Na2HPO4/ KH2PO4