5ECP
Crystal Structure of FIN219-FIP1 complex with JA, MET and ATP
Experimental procedure
Experimental method | LAUE |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-08 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97620-0.97622 |
Spacegroup name | P 1 |
Unit cell lengths | 53.708, 53.701, 192.029 |
Unit cell angles | 89.96, 90.16, 113.24 |
Refinement procedure
Resolution | 23.347 - 2.250 |
R-factor | 0.185207158756 |
Rwork | 0.185 |
R-free | 0.20767 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4epl |
RMSD bond length | 0.015 |
RMSD bond angle | 1.639 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.5.6) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.131 | 0.406 |
Number of reflections | 89193 | |
<I/σ(I)> | 4.6 | 1.992 |
Completeness [%] | 93.6 | 96.7 |
Redundancy | 1.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 297 | 0.2 M sodium acetate trihydrate, 0.1 M Tris-HCl, 30%(w/v) PEG 3000 |