5ECJ
Crystal structure of monobody Mb(S4) bound to Prdm14 in complex with Mtgr1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-13 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 106.812, 106.812, 180.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.764 - 3.050 |
R-factor | 0.1939 |
Rwork | 0.189 |
R-free | 0.24960 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.074 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX (1.9_1692) |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.764 | 3.160 |
High resolution limit [Å] | 3.050 | 3.050 |
Number of reflections | 20631 | |
<I/σ(I)> | 30 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 20 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 17% PEG3350, 8% Tacsimate |