5EC6
The apo crystal structure of haemoglobin receptor HpuA from Kingella denitrificans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.008 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 102.470, 102.470, 77.210 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.457 - 1.600 |
| R-factor | 0.1719 |
| Rwork | 0.171 |
| R-free | 0.19600 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.608 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 72.457 | 26.418 | 1.690 |
| High resolution limit [Å] | 1.600 | 5.060 | 1.600 |
| Rmerge | 0.058 | 0.853 | |
| Rmeas | 0.107 | ||
| Rpim | 0.032 | 0.019 | 0.262 |
| Total number of observations | 622672 | 20576 | 89805 |
| Number of reflections | 54716 | ||
| <I/σ(I)> | 12.6 | 25.9 | 2.8 |
| Completeness [%] | 100.0 | 99.3 | 100 |
| Redundancy | 11.4 | 10.7 | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 277 | 100 mM imidazole, 150 mM lithium sulfate, 6% Peg 3000 |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 277 | 100 mM imidazole, 150 mM lithium sulfate, 6% Peg 3000 |
