5EBL

KcsA T75G in the Conductive State

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	80
Detector technology	CMOS
Collection date	2015-03-12
Detector	RDI CMOS_8M
Wavelength(s)	1.0
Spacegroup name	I 4
Unit cell lengths	155.410, 155.410, 76.273
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.852 - 2.300
R-factor	0.2085
Rwork	0.205
R-free	0.24190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1k4c
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.8_1069)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	54.947	54.947	2.420
High resolution limit [Å]	2.300	7.270	2.300
Rmerge	0.109	0.034	1.291
Rmeas	0.118
Rpim	0.044	0.014	0.523
Total number of observations	290580	9581	41528
Number of reflections	40541
<I/σ(I)>	13.1	43.1	1.5
Completeness [%]	100.0	99.7	100
Redundancy	7.2	7.2	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	PEG 400