5EBI
Crystal structure of a DNA-RNA chimera in complex with Ba2+ ions: a case of unusual multi-domain twinning
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-08 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.8266 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.720, 44.100, 35.720 |
| Unit cell angles | 90.00, 119.98, 90.00 |
Refinement procedure
| Resolution | 30.940 - 1.090 |
| R-factor | 0.11361 |
| Rwork | 0.115 |
| R-free | 0.16900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3p4j |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.888 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.940 | 1.120 |
| High resolution limit [Å] | 1.090 | 1.090 |
| Rmerge | 0.071 | 0.375 |
| Number of reflections | 40052 | |
| <I/σ(I)> | 13.07 | 3.12 |
| Completeness [%] | 99.4 | 99.5 |
| Redundancy | 5.84 | 3.33 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 292 | A 1.5 MM WATER SOLUTION OF DNA-RNA MIXED 1:1 V/V WITH 10% MPD, 12 MM SPERMINE*4HCL, 80 MM NACL, 20 MM BACL2, 40 MM SODIUM CACODYLATE AND EQUILIBRATED AGAINST 35% MPD, PH 6.0 |
