5EBH

Crystal Structure HEW Lysozyme processed with the CrystalDirect automated mounting and cryo-cooling technology

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.966
Spacegroup name	P 43 21 2
Unit cell lengths	78.801, 78.801, 37.267
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.700 - 1.200
R-factor	0.17452
Rwork	0.173
R-free	0.20102
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4b0d
RMSD bond length	0.012
RMSD bond angle	1.900
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0131)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.700	1.220
High resolution limit [Å]	1.200	1.200
Rmerge	0.060
Number of reflections	37274
<I/σ(I)>	17.2
Completeness [%]	100.0
Redundancy	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	Crystallization experiments were carried out at the High Throughput Crystallization Laboratory of the EMBL Grenoble Outstation (https://embl.fr/htxlab) using the sitting-drop vapour diffusion method and CrystalDirect plates . Crystallization experiments were set up with 100 nl of sample and 100 nl of crystallization solution on the inner surface of the films within a CrystalDirect plate with a Cartesian PixSys robot (Cartesian Technologies). The crystallization solution was 0.1 M sodium acetate, pH=4.6 and 1.25 M sodium chloride.