5EB7

Crystal Structure of the Reversibly photoswitching chromoprotein Dathail, Metastable State

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2013-10-15
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.542
Spacegroup name	P 21 21 2
Unit cell lengths	76.250, 81.180, 39.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.180 - 1.650
R-factor	0.1857
Rwork	0.183
R-free	0.21680
RMSD bond length	0.006
RMSD bond angle	1.241
Data reduction software	MOSFLM
Phasing software	PHENIX
Refinement software	PHENIX (1.9pre_1665)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.180	1.709
High resolution limit [Å]	1.650	1.650
Rmerge	0.063	0.304
Number of reflections	29829
<I/σ(I)>	10.91	2.18
Completeness [%]	98.6	88.05
Redundancy	5.1	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M Magnesium Chloride, 0.1M Bis-Tris:HCl pH 6.5, 25% PEG 3350