5EAS
5-EPI-ARISTOLOCHENE SYNTHASE FROM NICOTIANA TABACUM
Experimental procedure
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 90 |
Detector technology | IMAGE PLATE |
Collection date | 1996-03 |
Detector | MARRESEARCH |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 124.730, 124.730, 118.940 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.250 |
R-factor | 0.24 |
Rwork | 0.240 |
R-free | 0.20000 |
Structure solution method | MIRAS |
RMSD bond length | 0.007 |
RMSD bond angle | 17.900 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | VECSUM |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.300 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.063 * | 0.275 * |
Total number of observations | 272509 * | |
Number of reflections | 42946 | |
<I/σ(I)> | 11 | 5.9 |
Completeness [%] | 95.2 | 96.7 |
Redundancy | 6.3 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.9 | PROTEIN WAS CRYSTALLIZED FROM 15% PEG 8000, 200 MM MG(OAC)2, 100 MM MOPSO PH 6.9, 1MM DTT; SOAKING SOLUTION FOR FREEZING ALSO INCLUDED 20% ETHYLENE GLYCOL. |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 15 (%) | |
2 | 1 | reservoir | 200 (mM) | ||
3 | 1 | reservoir | MOPSO | 100 (mM) | |
4 | 1 | reservoir | dithiothreitol | 1 (mM) |