5EAA

ASPARTATE AMINOTRANSFERASE FROM E. COLI, C191S MUTATION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	297
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS II
Spacegroup name	C 2 2 21
Unit cell lengths	156.270, 86.940, 79.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.400
R-factor	0.219
Rwork	0.219
Structure solution method	OTHER
Starting model (for MR)	1asa
RMSD bond length	0.018
RMSD bond angle	24.390 *
Data reduction software	R-AXIS
Data scaling software	R-AXIS
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400	2.400
Rmerge	0.072 *
Number of reflections	19539
Completeness [%]	92.0
Redundancy	1.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6 (mg/ml)
2	1	drop	potassium phosphate	20 (mM)
3	1	drop	PLP	0.01 (mM)
4	1	drop	EDTA	5 (mM)
5	1	reservoir	ammonium sulfate	40-54 (%)
6	1	reservoir	potassium phosphate	20 (mM)