5E9T
Crystal structure of GtfA/B complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2014-08-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9792 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 155.930, 196.410, 220.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 122.123 - 2.920 |
R-factor | 0.2199 |
Rwork | 0.217 |
R-free | 0.27500 |
Structure solution method | SAD |
Starting model (for MR) | 4pqg |
RMSD bond length | 0.010 |
RMSD bond angle | 1.346 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 122.123 | 122.100 | 2.670 |
High resolution limit [Å] | 2.920 | 11.340 | 2.600 |
Rmerge | 0.239 | 0.055 | |
Rmeas | 0.312 | 0.056 | |
Total number of observations | 5781655 | ||
Number of reflections | 73613 | 1363 | 7993 |
<I/σ(I)> | 23.13 | 77.62 | 0.2 |
Completeness [%] | 99.9 | 98.4 | 99.8 |
Redundancy | 58.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | PEG4000, magnesium chloride, Tris |