5E9K
Crystal Structure of BAZ2B bromodomain in complex with fragment F275
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-05 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.331, 96.181, 57.794 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.090 - 2.067 |
| R-factor | 0.1955 |
| Rwork | 0.194 |
| R-free | 0.21940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ir5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.077 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.090 | 48.090 | 2.180 |
| High resolution limit [Å] | 2.067 | 6.530 | 2.067 |
| Rmerge | 0.021 | 0.396 | |
| Rmeas | 0.054 | ||
| Rpim | 0.019 | 0.008 | 0.151 |
| Total number of observations | 121784 | 4283 | 17150 |
| Number of reflections | 14219 | ||
| <I/σ(I)> | 26.3 | 76.2 | 6.1 |
| Completeness [%] | 99.6 | 99.7 | 97.8 |
| Redundancy | 8.6 | 8.4 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | PEG500MME (20%), PEG1000 (2%), PEG3350 (2%), PEG20000 (10%), MPD (2%) |
