5E9A
Crystal structure analysis of the cold-adamped beta-galactosidase from Rahnella sp. R3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-11-18 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 146.426, 106.831, 164.244 |
| Unit cell angles | 90.00, 109.00, 90.00 |
Refinement procedure
| Resolution | 138.448 - 2.561 |
| R-factor | 0.2 |
| Rwork | 0.199 |
| R-free | 0.24680 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.555 |
| Data scaling software | SCALA (3.3.20) |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 138.448 | 138.448 | 2.700 |
| High resolution limit [Å] | 2.561 | 8.100 | 2.560 |
| Rmerge | 0.033 | 0.485 | |
| Rmeas | 0.163 | ||
| Rpim | 0.085 | 0.023 | 0.349 |
| Total number of observations | 535514 | 18074 | 69030 |
| Number of reflections | 151929 | ||
| <I/σ(I)> | 8.6 | 22.3 | 2.4 |
| Completeness [%] | 98.6 | 98.5 | 96.8 |
| Redundancy | 3.5 | 3.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8 | 293 | 0.2 M sodium acetate, 0.1 M Tris-HCl, 30%(w/v) PEG 1500 |
