5E96
Crystal structure of aminoglycoside 6'-acetyltransferase type Ii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-10-31 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 64 |
| Unit cell lengths | 66.815, 66.815, 104.129 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n71 |
| RMSD bond length | 0.006 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.1) |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.033 | 0.206 |
| Number of reflections | 15426 | |
| <I/σ(I)> | 22.4 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.6 | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 40% ethylene glycol, 0.1 M phosphate-citrate pH 4.2, 0.1 M ammonium sulfate |
