5E96
Crystal structure of aminoglycoside 6'-acetyltransferase type Ii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2006-10-31 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.979 |
Spacegroup name | P 64 |
Unit cell lengths | 66.815, 66.815, 104.129 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 2.100 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1n71 |
RMSD bond length | 0.006 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (1.3.1) |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.033 | 0.206 |
Number of reflections | 15426 | |
<I/σ(I)> | 22.4 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 9.6 | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 40% ethylene glycol, 0.1 M phosphate-citrate pH 4.2, 0.1 M ammonium sulfate |