5E8H
Crystal structure of geranylfarnesyl pyrophosphate synthases 2 from Arabidopsis thaliana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2013-10-08 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.987 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.245, 77.090, 114.172 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.520 - 2.300 |
R-factor | 0.2082 |
Rwork | 0.207 |
R-free | 0.23370 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.473 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.300 |
Number of reflections | 28044 |
<I/σ(I)> | 28.3 |
Completeness [%] | 90.1 |
Redundancy | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 2.0 M ammonium sulfate, 2% PEG 400, pH7.5 |