5E8C
pseudorabies virus nuclear egress complex, pUL31, pUL34
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2015-02-18 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97902 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 91.690, 91.690, 108.260 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 69.970 - 2.900 |
R-factor | 0.2216 |
Rwork | 0.219 |
R-free | 0.26470 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 0.900 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | BUSTER-TNT (BUSTER 2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.000 | 2.980 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.308 | 3.460 |
Number of reflections | 10771 | |
<I/σ(I)> | 35.6 | 4.2 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 227 | 209.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 16% PEGG6000, MMT buffer system pH7.0, 1M Lithium Chloride |