5E8C
pseudorabies virus nuclear egress complex, pUL31, pUL34
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2015-02-18 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97902 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 91.690, 91.690, 108.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.970 - 2.900 |
| R-factor | 0.2216 |
| Rwork | 0.219 |
| R-free | 0.26470 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.900 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | BUSTER-TNT (BUSTER 2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.980 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.308 | 3.460 |
| Number of reflections | 10771 | |
| <I/σ(I)> | 35.6 | 4.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 227 | 209.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 16% PEGG6000, MMT buffer system pH7.0, 1M Lithium Chloride |
