5E8B

Crystal structure of the S. cerevisiae Rtf1 histone modification domain mutant R126A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2014-02-06
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.1
Spacegroup name	H 3 2
Unit cell lengths	93.868, 93.868, 75.344
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.451 - 1.620
R-factor	0.1489
Rwork	0.147
R-free	0.19260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5emx
RMSD bond length	0.008
RMSD bond angle	0.867
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.451	1.650
High resolution limit [Å]	1.620	1.620
Rmerge	0.057	0.503
Number of reflections	16294
<I/σ(I)>	43	6.77
Completeness [%]	99.6	100
Redundancy	20	15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M phosphate-citrate (pH 4.2), 28% PEG 300, Silver Bullets Bio condition 7 (Hampton)