5E81
Structure of T. thermophilus 70S ribosome complex with mRNA and tRNALys in the A-site with wobble pair
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 209.200, 448.500, 619.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 147.011 - 2.950 |
| R-factor | 0.1959 |
| Rwork | 0.196 |
| R-free | 0.24180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wq1 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.054 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 170.000 | 3.030 | |
| High resolution limit [Å] | 2.950 | 13.190 | 2.950 |
| Rmerge | 0.319 | 0.046 | 5.191 |
| Rmeas | 0.322 | 0.046 | 5.237 |
| Total number of observations | 67576455 | ||
| Number of reflections | 1208590 | 14289 | 88967 |
| <I/σ(I)> | 15.38 | 85 | 1.03 |
| Completeness [%] | 100.0 | 99.5 | 100 |
| Redundancy | 55.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 20000, PEG 550mme, KSCN, Tris-OAc |
