5E7U

MBP-MamC loop structure, a magnetite biomineralizing protein from Magnetospirillium magneticum AMB-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2013-02-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.939
Spacegroup name	P 43 21 2
Unit cell lengths	66.600, 66.600, 211.137
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.520 - 2.800
R-factor	0.26958
Rwork	0.267
R-free	0.30725
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3puz
RMSD bond length	0.004
RMSD bond angle	0.837
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.520
High resolution limit [Å]	2.800
Rmerge	0.147	0.566
Number of reflections	11938
<I/σ(I)>	24.64	4.88
Completeness [%]	95.2
Redundancy	7.2	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M sodium acetate , 2 M Ammonium sulfate