5E7U
MBP-MamC loop structure, a magnetite biomineralizing protein from Magnetospirillium magneticum AMB-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-02-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.939 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 66.600, 66.600, 211.137 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.520 - 2.800 |
R-factor | 0.26958 |
Rwork | 0.267 |
R-free | 0.30725 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3puz |
RMSD bond length | 0.004 |
RMSD bond angle | 0.837 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 63.520 | |
High resolution limit [Å] | 2.800 | |
Rmerge | 0.147 | 0.566 |
Number of reflections | 11938 | |
<I/σ(I)> | 24.64 | 4.88 |
Completeness [%] | 95.2 | |
Redundancy | 7.2 | 8.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M sodium acetate , 2 M Ammonium sulfate |