5E7N

Crystal Structure of RPA70N in complex with VU0085636

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-05
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.978
Spacegroup name	P 21 21 21
Unit cell lengths	38.560, 53.260, 54.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.470 - 1.210
R-factor	0.1968
Rwork	0.196
R-free	0.21550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r4t
RMSD bond length	0.006
RMSD bond angle	1.206
Data reduction software	HKL-2000 (708)
Data scaling software	HKL-2000 (708)
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	31.470
High resolution limit [Å]	1.210
Number of reflections	34513
<I/σ(I)>	22.49
Completeness [%]	99.8
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	294	100 mM MES, 200 mM Calcium Acetate, 20% PEG 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K