5E7L
Crystal structure of mouse CNTN2 FN1-FN3 domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-24 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 124.390, 40.674, 82.599 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.103 - 2.002 |
| R-factor | 0.1926 |
| Rwork | 0.191 |
| R-free | 0.22640 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.059 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.110 | 0.060 | 0.508 |
| Total number of observations | 166345 | ||
| Number of reflections | 28357 | ||
| <I/σ(I)> | 10.8 | ||
| Completeness [%] | 97.8 | 99.6 | 89.3 |
| Redundancy | 5.9 | 6.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 50 mM NH4Cl, 50 mM Tris-HCl pH 8.5, 10% (w/v) PEG 4000, 2% (v/v) glycerol |
