5E7G
Crystal structure of Bacova_02650 with xylogluco-oligosaccharide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | H 3 2 |
Unit cell lengths | 207.390, 207.390, 117.870 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.190 - 2.370 |
R-factor | 0.199 |
Rwork | 0.198 |
R-free | 0.22600 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.905 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 39.190 |
High resolution limit [Å] | 2.370 |
Number of reflections | 39362 |
<I/σ(I)> | 20.9 |
Completeness [%] | 99.9 |
Redundancy | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 297 | 1.1 to 1.5 M ammonium sulphate, 30 to 70 mM sodium cacodylate pH 6.5. To decrease crystal nucleation, 0.3 ml of paraffin oil was overlaid on top of 0.5 mls of mother liquor |