5E7G
Crystal structure of Bacova_02650 with xylogluco-oligosaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 207.390, 207.390, 117.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.190 - 2.370 |
| R-factor | 0.199 |
| Rwork | 0.198 |
| R-free | 0.22600 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.905 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 39.190 |
| High resolution limit [Å] | 2.370 |
| Number of reflections | 39362 |
| <I/σ(I)> | 20.9 |
| Completeness [%] | 99.9 |
| Redundancy | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 297 | 1.1 to 1.5 M ammonium sulphate, 30 to 70 mM sodium cacodylate pH 6.5. To decrease crystal nucleation, 0.3 ml of paraffin oil was overlaid on top of 0.5 mls of mother liquor |
