5E7B
Structure of a nanobody (vHH) from camel against phage Tuc2009 RBP (BppL, ORF53)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-06 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.86 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 52.080, 52.080, 162.690 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.970 - 1.100 |
R-factor | 0.1595 |
Rwork | 0.158 |
R-free | 0.18920 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.367 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.100 | 1.130 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.068 | 1.550 |
Number of reflections | 52014 | |
<I/σ(I)> | 18.4 | 1.5 |
Completeness [%] | 96.6 | 91.7 |
Redundancy | 19.5 | 19.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | mixing 100-300 nl of 12 mg.mL-1 protein (Na2HPO4, 10 mM; KH2PO4, 1.8 mM, pH7.4; NaCl, 137 mM; KCl, 2.7mM) with 100 nl precipitant solution in 25-30 % PEG4000, 0.2 M Imidazole (pH 6.0). |