5E71
Crystal structure of the archaeal tRNA m2G/m22G10 methyltransferase (aTrm11) from Thermococcus kodakarensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 47.733, 53.520, 134.859 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.442 - 1.700 |
R-factor | 0.2065 |
Rwork | 0.205 |
R-free | 0.23950 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.027 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1682) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.730 |
High resolution limit [Å] | 1.700 | 4.610 | 1.700 |
Rmerge | 0.051 | 0.027 | 0.594 |
Rmeas | 0.055 | 0.029 | 0.641 |
Rpim | 0.021 | 0.011 | 0.238 |
Total number of observations | 275794 | ||
Number of reflections | 38847 | ||
<I/σ(I)> | 17 | ||
Completeness [%] | 99.8 | 97.4 | 100 |
Redundancy | 7.1 | 6.6 | 7.2 |
CC(1/2) | 0.999 | 0.951 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 4.5M NaCl, 0.1M Tris-HCl |