5E6Z
Crystal structure of Ecoli Branching Enzyme with beta cyclodextrin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-15 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97876 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 91.952, 102.995, 186.239 |
Unit cell angles | 90.00, 91.57, 90.00 |
Refinement procedure
Resolution | 44.927 - 1.878 |
R-factor | 0.2106 |
Rwork | 0.206 |
R-free | 0.24890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1m7x |
RMSD bond length | 0.007 |
RMSD bond angle | 1.095 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.878 |
<I/σ(I)> | 25 |
Completeness [%] | 99.5 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.2 | 277 | 0.1 M NaHEPES, pH = 7.2 |