5E6Y

Crystal structure of E.Coli branching enzyme in complex with alpha cyclodextrin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-20
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97876
Spacegroup name	P 1 21 1
Unit cell lengths	92.024, 103.808, 186.080
Unit cell angles	90.00, 91.97, 90.00

Refinement procedure

Resolution	42.431 - 2.600
R-factor	0.1971
Rwork	0.190
R-free	0.25570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m7x
RMSD bond length	0.009
RMSD bond angle	1.267
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600
<I/σ(I)>	25
Completeness [%]	95.0
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	277	0.1M HEPES, pH 7.2