5E6T
Crystal structure of bovine norovirus P domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.920, 63.860, 87.560 |
| Unit cell angles | 90.00, 115.06, 90.00 |
Refinement procedure
| Resolution | 40.810 - 2.270 |
| R-factor | 0.1971 |
| Rwork | 0.195 |
| R-free | 0.24120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zl5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.783 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.3) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.810 | 2.280 | |
| High resolution limit [Å] | 2.200 | 9.830 | 2.200 |
| Rmerge | 0.139 | 0.054 | 0.641 |
| Rmeas | 0.155 | 0.060 | 0.756 |
| Total number of observations | 144036 | ||
| Number of reflections | 28691 | 346 | 1959 |
| <I/σ(I)> | 10.53 | 28.73 | 2.79 |
| Completeness [%] | 98.9 | 97.2 | 92.1 |
| Redundancy | 5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | PEG 3350, Zinc acetate, imidazole |
