5E6R
Structures of leukocyte integrin aLb2: The aI domain, the headpiece, and the pocket for the internal ligand
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2012-04-07 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 1.03321 |
Spacegroup name | P 61 |
Unit cell lengths | 153.929, 153.929, 116.454 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.242 - 2.901 |
R-factor | 0.1957 |
Rwork | 0.193 |
R-free | 0.22890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4neh |
RMSD bond length | 0.012 |
RMSD bond angle | 0.788 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.000 |
Rmerge | 0.093 |
Number of reflections | 33981 |
<I/σ(I)> | 9.47 |
Completeness [%] | 99.9 |
Redundancy | 3.11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M MES pH 6.5, 15% PEG 3350 |