5E6P
PlexinB2 cytoplasmic region/PDZ-RhoGEF PDZ domain complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | .979237 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 126.152, 126.152, 211.465 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.530 - 3.215 |
| R-factor | 0.2562 |
| Rwork | 0.254 |
| R-free | 0.29660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ig3 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.754 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 16847 | |
| <I/σ(I)> | 56 | 1.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 30.1 | 27.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 293 | 0.2 M K/Na tartrate, 0.1 M Na citrate pH 5.3, 1.4 M ammonium sulfate |
