5E6G
Crystal Structure of De Novo Designed Protein CA01
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 63 |
| Detector technology | CCD |
| Collection date | 2014-06-14 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 |
| Unit cell lengths | 116.537, 116.537, 57.215 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.850 - 2.090 |
| R-factor | 0.202 |
| Rwork | 0.200 |
| R-free | 0.23400 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.616 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 58.270 |
| High resolution limit [Å] | 2.090 |
| Number of reflections | 17000 |
| <I/σ(I)> | 13.8 |
| Completeness [%] | 98.6 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293.15 | 0.1M Tris:HCL, pH 8.5 - 1.0M Ammonium Phosphate Dibasic |
