5E6F

Canarypox virus resolvase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-23
Detector	PSI PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 43 21 2
Unit cell lengths	89.117, 89.117, 117.945
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.600
Rwork	0.223
R-free	0.26430
Structure solution method	SAD
Starting model (for MR)	1kcf
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	89.120	89.120	2.510
High resolution limit [Å]	2.420	9.050	2.420
Rmerge	0.098	0.025	1.917
Rpim	0.042	0.012	0.804
Total number of observations	117127	2022	12313
Number of reflections	18653
<I/σ(I)>	14.5	48.9	1.4
Completeness [%]	99.7	94.7	99.9
Redundancy	6.3	5.1	6.4
CC(1/2)	0.999	0.999	0.611

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICRODIALYSIS	8.5	295	Crystals generally appeared after one week and continued to grow for another week to typical dimensions of 0.1 x 0.1 x 0.5 mm