5E6F
Canarypox virus resolvase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-23 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 89.117, 89.117, 117.945 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| Rwork | 0.223 |
| R-free | 0.26430 |
| Structure solution method | SAD |
| Starting model (for MR) | 1kcf |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 89.120 | 89.120 | 2.510 |
| High resolution limit [Å] | 2.420 | 9.050 | 2.420 |
| Rmerge | 0.098 | 0.025 | 1.917 |
| Rpim | 0.042 | 0.012 | 0.804 |
| Total number of observations | 117127 | 2022 | 12313 |
| Number of reflections | 18653 | ||
| <I/σ(I)> | 14.5 | 48.9 | 1.4 |
| Completeness [%] | 99.7 | 94.7 | 99.9 |
| Redundancy | 6.3 | 5.1 | 6.4 |
| CC(1/2) | 0.999 | 0.999 | 0.611 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 8.5 | 295 | Crystals generally appeared after one week and continued to grow for another week to typical dimensions of 0.1 x 0.1 x 0.5 mm |
