5E6A
Glucocorticoid receptor DNA binding domain - PLAU NF-kB response element complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-01 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.993, 95.497, 103.634 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.114 - 2.197 |
R-factor | 0.2389 |
Rwork | 0.236 |
R-free | 0.26580 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.842 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.230 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 20243 | |
<I/σ(I)> | 16.2 | 1.67 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.1 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.05 M sodium cacodylate, 0.05 M spermine, and 16% PEG 400 |