5E69

Glucocorticoid receptor DNA binding domain - IL8 NF-kB response element complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-12
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	39.165, 97.176, 103.265
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.267 - 1.850
R-factor	0.2212
Rwork	0.220
R-free	0.24180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.852
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.270	1.920
High resolution limit [Å]	1.850	1.850
Number of reflections	30412
<I/σ(I)>	14	2.8
Completeness [%]	88.4	74.7
Redundancy	4.4	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M HEPES pH 7.5, 7.5% glycerol, 22% PEG 20000