5E5X
Structure of the amyloid forming peptide ANFLVH (residues 13-18) from islet amyloid polypeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 291 |
| Detector technology | CCD |
| Collection date | 2009-11-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 4.831, 39.726, 9.869 |
| Unit cell angles | 90.00, 103.69, 90.00 |
Refinement procedure
| Resolution | 19.863 - 1.610 |
| R-factor | 0.1179 |
| Rwork | 0.113 |
| R-free | 0.16120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.704 |
| Data reduction software | DENZO |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.000 | 1.720 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.147 | |
| Number of reflections | 433 | |
| <I/σ(I)> | 6.55 | 9.7 |
| Completeness [%] | 93.3 | 87 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 20 mg/ml in water and mixed with 10% (w/v) PEG-8000, 0.1 M Na/K phosphate pH 6.2, and 0.2 M NaCl |
