5E5V
Structure of amyloid forming peptide NFGAILS (residues 22-28) from Islet Amyloid Polypeptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 291 |
Detector technology | CCD |
Collection date | 2013-03-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 |
Unit cell lengths | 8.661, 11.594, 21.552 |
Unit cell angles | 86.39, 82.21, 76.35 |
Refinement procedure
Resolution | 21.342 - 1.240 |
R-factor | 0.1759 |
Rwork | 0.173 |
R-free | 0.20620 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.024 |
Data reduction software | DENZO |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.340 |
High resolution limit [Å] | 1.240 | 1.280 |
Rmerge | 0.166 | 0.558 |
Number of reflections | 2283 | |
<I/σ(I)> | 6.48 | 1.6 |
Completeness [%] | 97.8 | 96.9 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 7 mg/ml in water and mixed with 10% ethanol and 1.5M NaCl |