5E55

Crystal structure of mouse CNTN6 FN1-FN3 domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-03-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	86.744, 90.847, 99.349
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.372 - 2.702
R-factor	0.2019
Rwork	0.200
R-free	0.24080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e4s
RMSD bond length	0.004
RMSD bond angle	0.917
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.155	0.074	0.515
Total number of observations	160904
Number of reflections	21656
<I/σ(I)>	8.4
Completeness [%]	97.9	96	92.5
Redundancy	7.4	8.1	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	300 mM Na-Malonate pH 7.0, 20% (w/v) PEG 3350, 50 mM Na-Cacodylate pH 6.5