5E4X
Crystal structure of cpSRP43 chromodomain 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.033 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 103.860, 103.860, 103.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.400 - 2.750 |
| R-factor | 0.2484 |
| Rwork | 0.247 |
| R-free | 0.27950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2n88 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.779 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.400 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.257 | 2.462 |
| Number of reflections | 2708 | |
| <I/σ(I)> | 23.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 48.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277.15 | 25% PEG 3350, 0.1 M Hepes pH 7.5, 0.2 M MgCl2 |
