Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-02-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 111.114, 111.114, 137.090 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 96.230 - 2.430 |
| R-factor | 0.17468 |
| Rwork | 0.173 |
| R-free | 0.20630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2W9I |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.089 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.230 | 2.520 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.050 | |
| Number of reflections | 35368 | |
| <I/σ(I)> | 34.8 | 5.5 |
| Completeness [%] | 99.9 | 97.2 |
| Redundancy | 5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 46% PEG200 (v/v) in 100 mM NaCl/1 mM MES, pH 6.5. |
