5E4L
Structure of ligand binding region of uPARAP at pH 5.3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.270, 102.700, 87.760 |
| Unit cell angles | 90.00, 94.54, 90.00 |
Refinement procedure
| Resolution | 54.430 - 2.440 |
| R-factor | 0.2194 |
| Rwork | 0.217 |
| R-free | 0.25880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qo6 1tdq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.020 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.430 | 2.530 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.085 | 0.710 |
| Number of reflections | 48904 | |
| <I/σ(I)> | 16.2 | 2.9 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.6 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.3 | 293 | 8-12% (w/v) PEG 3350, 200 mM NaCl, 35 mM CaCl2, 50 mM sodium acetate |
