5E4G
Crystal structure of human growth differentiation factor 11 (GDF-11)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-02-06 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 32.270, 32.270, 210.602 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.818 - 1.500 |
R-factor | 0.189 |
Rwork | 0.188 |
R-free | 0.20710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | homology model |
RMSD bond length | 0.006 |
RMSD bond angle | 1.058 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 22.820 | 22.820 | 1.550 |
High resolution limit [Å] | 1.500 | 3.230 | 1.500 |
Rmerge | 0.056 | 0.034 | 0.560 |
Total number of observations | 56267 | 5676 | 5275 |
Number of reflections | 17599 | ||
<I/σ(I)> | 9.4 | 31.7 | 1.9 |
Completeness [%] | 92.3 | 83.4 | 91.8 |
Redundancy | 3.11 | 3.11 | 3.01 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.18 M magnesium acetate, 0.1 M HEPES, pH 7.5, 22.5% PEG3350, 0.01 M strontium chloride |