5E43
Crystal Structure of Beta-lactamase Sros_5706 from Streptosporangium roseum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-03 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97918 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 74.845, 92.932, 85.629 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.146 - 1.710 |
R-factor | 0.1607 |
Rwork | 0.159 |
R-free | 0.18710 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 0.973 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | PHENIX ((1.10pre_2104: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.779 | |
Number of reflections | 32159 | |
<I/σ(I)> | 17.12 | 1.89 |
Completeness [%] | 99.8 | 97.9 |
Redundancy | 5.5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 289 | 6.0 M Ammonium nitrate, 0.1 M, Sodium acetate trihydrate pH 4.6 |