5E3U

Crystal structure of phosphatidylinositol-4-phosphate 5-kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.987
Spacegroup name	P 43 21 2
Unit cell lengths	88.134, 88.134, 155.757
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 3.600
R-factor	0.2036
Rwork	0.201
R-free	0.26070
RMSD bond length	0.011
RMSD bond angle	1.603
Data reduction software	HKL-2000
Refinement software	REFMAC (5.8.0071)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	3.600
Number of reflections	7655
<I/σ(I)>	5.5
Completeness [%]	100.0
Redundancy	13.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	peg