5E3T
Crystal structure of phosphatidylinositol-4-phosphate 5-kinase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.987 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 87.928, 87.928, 156.462 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 3.300 |
R-factor | 0.2074 |
Rwork | 0.204 |
R-free | 0.27230 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.746 |
Data reduction software | HKL-3000 |
Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.000 |
High resolution limit [Å] | 3.300 |
Number of reflections | 9831 |
<I/σ(I)> | 9.9 |
Completeness [%] | 99.5 |
Redundancy | 9.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | peg |