5E3S

Crystal structure of Phosphatidylinositol-4-phosphate 5-kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.987
Spacegroup name	P 43 21 2
Unit cell lengths	88.931, 88.931, 154.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.890 - 3.100
R-factor	0.2129
Rwork	0.210
R-free	0.27280
RMSD bond length	0.014
RMSD bond angle	1.863
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	3.100
Number of reflections	11844
<I/σ(I)>	11.3
Completeness [%]	100.0
Redundancy	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	Peg